Bookcover of Studies on minispectrins
Booktitle:

Studies on minispectrins

Expression in E. coli, purification and in vitro interaction studies

VDM Verlag Dr. Müller (2009-05-17 )

Books loader

Omni badge eligible for voucher
ISBN-13:

978-3-639-15735-2

ISBN-10:
3639157354
EAN:
9783639157352
Book language:
English
Blurb/Shorttext:
The α/β-minispectrin is a shortened soluble version of spectrin constructed to study spectrin interactions in vitro. In this study we constructed a modified α/β÷N-minispectrin, lacking the 37 amino acid N-terminal extension of the β-minispectrin chain. The original and modified version of the minispectrin was produced in order to perform structural and functional studies. Upon production of the α/β- and α/β÷N-minispectrin proteins, two versions of each protein were obtained, a dimer, (α/β)/ (α/β÷N), and a tetramer, (α/β)2/(α/β÷N)2. The effect of calcium on the binding of spectrin to actin has for long been a theme of dispute. Therefore the influence of calcium on the interaction between actin and the minispectrins were investigated using two different methods, an ultracentrifuge actin pull down assay and Biacore®X. The results from both methods showed that calcium had no direct effect on the binding of the minispectrins to the actin.
Publishing house:
VDM Verlag Dr. Müller
Website:
http://www.vdm-verlag.de
By (author) :
Elin Soerhus
Number of pages:
84
Published at:
2009-05-17
Stock:
Available
Category:
Biology
Price:
49.00 €
Keywords:
Minispectrin, Interaction, Actin, Calcium, RACK1, BIAcore, actin-pull-down assay

Books loader

Newsletter

Adyen::diners Adyen::jcb Adyen::discover Adyen::amex Adyen::mc Adyen::visa Adyen::cup Adyen::unionpay Adyen::paypal Paypal CryptoWallet Wire Transfer

  0 products in the shopping cart
Edit cart
Loading frontend
LOADING