Bookcover of Porous Silicon Platform for Investigation of Transmembrane Proteins
Booktitle:

Porous Silicon Platform for Investigation of Transmembrane Proteins

An Artificial Cell Membrane

LAP LAMBERT Academic Publishing (2013-05-02 )

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ISBN-13:

978-3-659-38542-1

ISBN-10:
3659385425
EAN:
9783659385421
Book language:
English
Blurb/Shorttext:
A method to fabricate porous silicon membranes as thin as 1 micrometer for analysis of transmembrane proteins is detailed. This comprehensive presentation includes: 1) The detailed process to fabricate porous silicon membranes that are supported from the sides. The porous silicon membrane acts as a sieve that separates two compartments. 2) A procedure to deposit a viable and robust synthetic lipid bilayer membrane on the porous silicon, and incorporate transmembrane proteins into it. This procedure includes answers to what technique to use for lipid bilayer deposition, and which lipids to choose for incorporating proteins. This lipid bilayer membrane acts as a plasma membrane in living cells. 3) A comprehensive analysis,characterization, and assessment of the complete process and each individual step is performed using electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM), and scanning electron microscopy (SEM). The rigid porous wall and the lipid membrane with fused functional transmembrane proteins result in a device that resembles the cellular wall of the E. Coli bacteria. Numerous membrane proteins may be analyzed using this device.
Publishing house:
LAP LAMBERT Academic Publishing
Website:
https://www.lap-publishing.com/
By (author) :
Khalid Hasan Tantawi
Number of pages:
140
Published at:
2013-05-02
Stock:
Available
Category:
Electronics, electro-technology, communications technology
Price:
61.90 €
Keywords:
Porous silicon, Transmembrane Proteins, Lipid bilayer membranes, Electrochemical impedance spectroscopy

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