Bookcover of Expression, purification and cellular uptake of a S100A4 protein
Booktitle:

Expression, purification and cellular uptake of a S100A4 protein

an agent promoting metastasis

VDM Verlag Dr. Müller (2009-07-03 )

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ISBN-13:

978-3-639-17306-2

ISBN-10:
3639173066
EAN:
9783639173062
Book language:
English
Blurb/Shorttext:
A recombinant S100A4 protein was produced in Pichia pastoris (Pichia). The expression vector contained parts of the AOX1 promoter and also a secretion signal, making it possible to purify the protein from the yeast media after methanol induction. Attempts were made to purify the protein from the media both by hydrophobic interaction chromatography (HIC) and by anionic ion exchange chromatography (IEC). The binding capacity to the hydrophobic material was rather low, but the eluted protein did however show an acceptable purity for utilization in downstream functional assays. The protein eluted from the hydrophobic column was labelled with the fluorescent dye (Cy3) and used for internalization studies in the human cell line HeLa. Similar to a mouse rS100A4 produced in bacterial cells, the Pichia produced protein was also found internalized into the HeLa cells. The biological significance of these findings is at present not elucidated and will be further studied.
Publishing house:
VDM Verlag Dr. Müller
Website:
http://www.vdm-verlag.de
By (author) :
stian sjøli
Number of pages:
152
Published at:
2009-07-03
Stock:
Available
Category:
Biochemistry, biophysics
Price:
59.00 €
Keywords:
Recombinant protein, S100A4, cancer

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