Bookcover of CHEMICAL MECHANISM OF HOMOCITRATE SYNTHASE FROM Saccharomyces cerevisiae
Booktitle:

CHEMICAL MECHANISM OF HOMOCITRATE SYNTHASE FROM Saccharomyces cerevisiae

VDM Verlag Dr. Müller (2009-05-31 )

Books loader

Omni badge eligible for voucher
ISBN-13:

978-3-639-16155-7

ISBN-10:
3639161556
EAN:
9783639161557
Book language:
English
Blurb/Shorttext:
Homocitrate synthase (HCS1) (acetyl-coenzyme A: 2-ketoglutarate C-transferase; E.C. 2.3.3.14) catalyzes the first and regulated step in the a-aminoadipate pathway for lysine synthesis. The pH dependence of the kinetic parameters, isotope effects, and dissociation constants for competitive inhibitors are used to probe the chemical mechanism of HCS. A general acid-base chemical mechanism is proposed. site-directed mutagenesis was used to change the three active site residues of HCS, and the resulting mutant enzymes were characterized using initial velocity studies, the pH dependence of the kinetic parameters and isotope effects. Data combined with a constant pH molecular dynamics simulation study suggest a catalytic dyad, comprised of Glu-155 and His-309, functions as a general base to deprotonate the methyl group of AcCoA.
Publishing house:
VDM Verlag Dr. Müller
Website:
http://www.vdm-verlag.de
By (author) :
jinghua qian
Number of pages:
164
Published at:
2009-05-31
Stock:
Available
Category:
Biochemistry, biophysics
Price:
68.00 €
Keywords:
Homocitrate synthase, Chemical mechanism, pH-rate profile, site-direct mutagenesis, inhibition study

Books loader

Newsletter

Adyen::diners Adyen::jcb Adyen::discover Adyen::amex Adyen::mc Adyen::visa Adyen::cup Adyen::alipay Adyen::unionpay Adyen::paypal Paypal CryptoWallet Wire Transfer

  0 products in the shopping cart
Edit cart
Loading frontend
LOADING