Bookcover of Somatic Embryogenesis and Genetic Transformation in Sugarcane
Booktitle:

Somatic Embryogenesis and Genetic Transformation in Sugarcane

Plant Regeneration through Somatic Embryogenesis in Sugarcane and its Transformation

LAP LAMBERT Academic Publishing (2012-06-16 )

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ISBN-13:

978-3-659-15388-4

ISBN-10:
3659153885
EAN:
9783659153884
Book language:
English
Blurb/Shorttext:
An efficient protocol for somatic embryogenesis and subsequent plant regeneration was developed for sugarcane variety Isd-16 using leaf sheath explants of 3, 6 and 12-months-old field grown plants. Explants from 6-month-old plant showed the best response and produced highest percentage of calli on MS + 3.0 mg/l 2,4-D. L-proline 25mg/l significantly enhanced somatic embryogenesis. The embryos germinated well on half-strength MS and developed into plantlets. Somatic embryo derived plants under field condition showed considerable variation in morphological, agronomical and biochemical characters. For genetic transformation the calli were co-cultivated with A. tumefaciens strain LBA4404 harboring a binary plasmid pCAMBIA1303 containing hpt (hygromycin phosphotransferase) gene as a selectable marker and a β-glucuronidase (gus) reporter gene in the T-DNA region. The transient expression of gus in hygromycin resistant calli and regenerated plants was confirmed by GUS flurometric assay. PCR analysis of genomic DNA from regenerated plants revealed that the hpt gene was integrated in the transgenic plants.
Publishing house:
LAP LAMBERT Academic Publishing
Website:
https://www.lap-publishing.com/
By (author) :
Mohashweta Roy, Monzur Hossain, Rafiul Islam
Number of pages:
164
Published on:
2012-06-16
Stock:
Available
Category:
Botany
Price:
68.00 €
Keywords:
Sugarcane, callus induction, plant regeneration, Genetic Transformation, Agrobacterium, Somatic embryogenesis

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