Bookcover of Putative Macaque and Orangutan homologues of tripeptidyl-peptidase II
Booktitle:

Putative Macaque and Orangutan homologues of tripeptidyl-peptidase II

underline the importance of its C-terminal part for enzymatic activity and proper oligomerization

AV Akademikerverlag (2013-05-02 )

Books loader

Omni badge eligible for voucher
ISBN-13:

978-3-639-46765-9

ISBN-10:
3639467655
EAN:
9783639467659
Book language:
English
Blurb/Shorttext:
Tripeptidyl-peptidase II (TPP2) is a 138–150 kDa serine protease with a subtilisin-like N-terminal catalytic domain and an 800 residues long C-terminal extension. It releases tripeptides from the N-terminus of its substrates and has been implicated in various processes, e. g. general protein turnover and MHC I antigen processing. TPP2 has been observed in different oligomeric states, ranging from dimers up to tetracontamers. Intriguingly, its specific activity increases with oligomer size. Although oligomerization and oligomerization-dependent activity enhancement remain poorly understood, they likely involve the C-terminal part of TPP2. To validate this assumption, we investigated two shortened homologues of human TPP2: mTPP2 from M. fascicularis and oTPP2 from P. abelii lacked the complete C-terminal segment and about half of it, respectively. We partially succeeded in expressing tagged constructs of these putative enzymes in E. coli and in purifying them from bacterial cell lysates. Both proteins appeared highly polydisperse during analytical gel filtration and were inactive against a standard substrate. These findings suggest that mTPP2 and oTPP2 represent truncated variants of their respective full-length sequences, and that proper oligomerization requires a complete C-terminal part.
Publishing house:
AV Akademikerverlag
Website:
http://www.akademikerverlag.de/
By (author) :
Wolfgang Skala
Number of pages:
184
Published on:
2013-05-02
Stock:
Available
Category:
Biochemistry, biophysics
Price:
42.95 €
Keywords:
Sumatran Orangutan, tripeptidyl-peptidase II, Cynomolgus monkey, enzyme characterization, Protein Expression, protease

Books loader

Adyen::diners Adyen::jcb Adyen::discover Adyen::amex Adyen::mc Adyen::visa Adyen::cup Adyen::unionpay Adyen::paypal Paypal Wire Transfer

  0 products in the shopping cart
Edit cart
Loading frontend
LOADING