Bookcover of Monoclonal antibodies anti-beta2-glycoprotein I
Booktitle:

Monoclonal antibodies anti-beta2-glycoprotein I

Production, characterization, analytical applications

LAP LAMBERT Academic Publishing (2013-05-13 )

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ISBN-13:

978-3-659-39385-3

ISBN-10:
3659393851
EAN:
9783659393853
Book language:
English
Blurb/Shorttext:
Beta2-glycoprotein I (β2GPI) detection is important for the accurate diagnosis of autoimmune and chronic inflammatory disease. This book describes the main steps towards a successful obtaining, initial characterization, analytical applications and lab-scale production of a set of anti-β2GPI monoclonal antibodies. The β2GPI antigen purification was comprehensively explored and six different hybridomas were obtained after cell fusion of immunized mouse lymphocytes with SP2-O murine myeloma cells. The antibodies were purified after hybridomas expansion from ascites (in vivo) and conventional cell culture (in vitro) and their reactivity was evaluated towards the β2GPI antigen either adsorbed to solid phase support (Dot-Blot, immunoblotting, direct ELISA), or in solution (indirect ELISA). β2GPI recognition was achieved with one of the clones in paraffin embedded tissues (immunohistochemistry), and in blood and lymph (ELISA). The application of β2GPI recognition to a sepsis and bacterial translocation animal model granted an unexpected and exciting sequence of achievements concerning the pathophysiology of autoimmunity control by this protein.
Publishing house:
LAP LAMBERT Academic Publishing
Website:
https://www.lap-publishing.com/
By (author) :
Carolina Nigro Stella, Lígia Ferreira Gomes
Number of pages:
92
Published on:
2013-05-13
Stock:
Available
Category:
Clinical disciplines
Price:
29.90 €
Keywords:
cell culture, protein purification, Immunohistochemistry, ELISA, monoclonal antibodies, Affinity Chromatography, hybridoma, beta2-glycoprotein I, immunoblotting, immune complexes

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