Bookcover of Mitochondrial Alterations in Solid Carcinomas
Booktitle:

Mitochondrial Alterations in Solid Carcinomas

Relationship between Tumorgenesis of Human Kidney Carcinomas and Mitochondrial DNA (mtDNA) Alterations

VDM Verlag Dr. Müller (2008-03-27 )

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ISBN-13:

978-3-8364-8640-8

ISBN-10:
3836486407
EAN:
9783836486408
Book language:
English
Blurb/Shorttext:
Alterations of the respiratory chain and mutations of the mitochondrial DNA (mtDNA) have been extensively investigated in a wide variety of neoplasias. However, no obvious correlation of a certain type of tumor and mtDNA mutations has been reported so far. I compared both, enzymatic measurements of the respiratory chain and mtDNA mutations in renal carcinomas and renal oncocytomas. To enable a high throughput screen of mtDNA variations, I developed a DHPLC-based protocol for a rapid detection of unknown variations of the entire human mtDNA. The benign renal oncocytomas were in clear contrast to the malign renal carcinomas. The oncocytomas showed significantly increased Krebs cycle- and OXPHOS enzyme activities, except a total breakdown of the complex I enzyme activity. The mutation screening revealed frame shift mutations, encoding mitochondrial ND genes in 8 out of 12 oncocytomas. All these somatic frame shift mutations displayed a homoplasmy of 100%. This study shows for the first time a direct correlation of mtDNA mutations in a specific type of cancer, which leads to a total lack of complex I enzyme activity in the respiratory chain.
Publishing house:
VDM Verlag Dr. Müller
Website:
http://www.vdm-verlag.de
By (author) :
David Meierhofer
Number of pages:
112
Published on:
2008-03-27
Stock:
Available
Category:
Biochemistry, biophysics
Price:
49.00 €
Keywords:
mitochondria, Mutation, cancer, OXPHOS, renal oncocytoma, renal carcinoma, mtDNA mutations, DHPLC, leukemia

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