Bookcover of Isolation of a promoter-specific chromatin remodeling factor
Booktitle:

Isolation of a promoter-specific chromatin remodeling factor

Scholars' Press (2013-08-03 )

Books loader

Omni badge eligible for voucher
ISBN-13:

978-3-639-51701-9

ISBN-10:
3639517016
EAN:
9783639517019
Book language:
English
Blurb/Shorttext:
Nucleosomes inhibit transcription by preventing the binding of RNA polymerase II and the general transcription factors to promoter DNA. This inhibition is relieved when promoter chromatin is remodeled to render the DNA accessible for transcription. Here we show that the chromatin remodeling factor Chd1 selectively remodels promoter nucleosomes. Chromatin circles of the repressed PHO5 gene, containing both promoter and open reading frame (ORF), were remodeled by yeast extract in a gene activator-dependent, ATP-dependent manner. The product of the reaction bore the hallmark of the process in vivo, the selective removal of promoter nucleosomes, without effect on ORF nucleosomes. Fractionation of the extract identified a single protein, Chd1, capable of the remodeling activity. Our results show that a single enzyme, Chd1, can distinguish between native promoter and ORF nucleosomes. In agreement with previous observations in yeast and in higher eukaryotes, we speculate that Chd1 may recognize histone marks specific to promoters and play a general role in gene activation via the establishment of open promoter chromatin.
Publishing house:
Scholars' Press
Website:
http://www.scholars-press.com
By (author) :
Andreas Ehrensberger
Number of pages:
132
Published on:
2013-08-03
Stock:
Available
Category:
Biochemistry, biophysics
Price:
67.90 €
Keywords:
Transcription, Biochemistry, Chromatin, chd1, nucleosomes

Books loader

Newsletter

Adyen::diners Adyen::jcb Adyen::discover Adyen::amex Adyen::mc Adyen::visa Adyen::cup Adyen::unionpay Adyen::paypal Paypal CryptoWallet Wire Transfer

  0 products in the shopping cart
Edit cart
Loading frontend
LOADING