Bookcover of Genetic transformation of rice with gshf for abiotic stress tolerance
Booktitle:

Genetic transformation of rice with gshf for abiotic stress tolerance

LAP LAMBERT Academic Publishing (2016-10-25 )

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ISBN-13:

978-3-659-97359-8

ISBN-10:
3659973599
EAN:
9783659973598
Book language:
English
Blurb/Shorttext:
Rice (Oryza sativa L.) is a staple food crop which greatly affected by different environmental stresses such as drought, salt and low temperature. Glutathione gshF, the tripeptide is a multifunctional metabolite in plants. A novel bifunctional enzyme from the Listeria monocytogenes), has been used to develop transgenic rice plants under 35s CaMV promoter in the background of indica rice cultivar NAUR-1 through Agrobacterium-mediated transformation. Total of 200 scutellum-derived embryogenic calli was used for transformation, screened by PCR namely gshF-5, gshF-12 and gshF-19, were obtained with 1.5% transformation frequency. The gshF gene was stably inherited and expressed in T2 plants, as indicated by the results of PCR, ELISA, RT-PCR and qRT-PCR analyses. Average expression of gshF was 3.03 µg/mg of total soluble protein in leaf extracts of transgenic plants. qRT-PCR analysis of gshF showed 5.83 and 6.07 higher fold in gshF-5-7 and gshF-5-8 at 200 mM NaCl treatment as compare to control. In transformed plant, glutathione content was increased more than 14 fold as compared to untransformed plant at 200 mM NaCl treatment.
Publishing house:
LAP LAMBERT Academic Publishing
Website:
https://www.lap-publishing.com/
By (author) :
Vishal Srivashtav, Sanjay Jha, Vipul Parekh
Number of pages:
164
Published on:
2016-10-25
Stock:
Available
Category:
Agriculture, horticulture, forestry, fishery, nutrition
Price:
64.90 €
Keywords:
Abiotic Stress Tolerance, Glutathione, Real Time, salt stress, Transgenic rice, Multifunctional metabolite

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