Couverture de Visualizing the Trafficking of Transport Vesicles in Neurons
Titre du livre:

Visualizing the Trafficking of Transport Vesicles in Neurons

Scholars' Press (23-12-2014 )

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ISBN-13:

978-3-639-76085-9

ISBN-10:
3639760859
EAN:
9783639760859
Langue du livre:
Anglais
texte du rabat:
In neurons transmembrane proteins are targeted to dendrites in vesicles that traffic solely within the somatodendritic compartment. How these vesicles are retained within the somatodendritic domain is unknown. Here we adapt a novel pulse chase system that allows synchronous release of exogenous transmembrane proteins from the endoplasmic reticulum using FKBP12 and Rapamycin. We demonstrate proof-of-concept and establish protein trafficking controls in incremental steps. We demonstrate the utility of this approach in studying protein trafficking and establish parameters for analysis of time-lapse images. We implement this novel pulse-chase strategy to track the movements of post-Golgi transport vesicles. Surprisingly, we found that post-Golgi vesicles carrying dendritic proteins were equally likely to enter axons and dendrites. However, once such vesicles entered the axon they very rarely moved beyond the axon initial segment, but instead either halted or reversed direction in an actin and Myosin Va-dependent manner.
Maison d'édition:
Scholars' Press
Site Web:
http://www.scholars-press.com
de (auteur) :
Sarmad Al-Bassam
Numéro de pages:
96
Publié le:
23-12-2014
Stock:
Disponible
Catégorie:
Biologie
Prix:
59.90 €
Mots-clés:
Dendrites, Trafficking, vesicles, axon initial segment

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