Fungal Proteomics of Ophiostoma ulmi的封面
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Fungal Proteomics of Ophiostoma ulmi

Proteomic Investigation of Biosynthesis Exopolysaccharide by Ophiostoma ulmi

LAP LAMBERT Academic Publishing (2010-05-30 )

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ISBN-13:

978-3-8383-0501-1

ISBN-10:
3838305019
EAN:
9783838305011
书籍语言:
英文
作品简介:
The dutch elm tree disease casual agent, Ophiostoma ulmi, was investigated for its ability to produce exopolyssacharides (EPS), which have great industrial potential. This book introduced the study of EPS biosynthesis, using proteomics tools with the purpose of identifying the proteins/enzymes that were responsible for EPS biosynthesis. The extracellular O. ulmi protein profile under various culture conditions was obtained with SDS PAGE and then compared. The gel bands were then excised and treated with trypsin, followed by protein identification by a matrix assisted laser desorption/ionization time of flight (MALDI-TOF) instrument. It was found that sucrose synthase, acyl carriers proteins and various transferases were induced by substrate (sucrose), or nitrogen source (asparagine), which indicated an intracellular biosynthesis pathway. This is the first proteomic work based on O. ulmi, which provides insights on both fungal proteomics and fungal EPS biosynthesis.
出版社 :
LAP LAMBERT Academic Publishing
网址:
https://www.lap-publishing.com/
由(作者):
Chun Bei Huang, and Bradley A. Saville
页码 :
100
发表日期:
2010-05-30
现货:
备有现货
类别:
微生物学
价格:
49.00 €
关键词:
fungal proteomics, Ophiostoma ulmi, biosynthesis, Exopolysaccharide (EPS), matrix assisted laser desorption/ionization time of flight (MALDI-TOF), intracellular biosynthesis, Biosynthesis, matrix assisted laser desorption/ionization time of flight (MALDI-TOF), intracellular biosynthesis

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